THE SYSTEM ARCHITECTURE AND METHODOLOGY FOR LIDAR AND CAMERA FUSION

Master'sMASTER OF ENGINEERIN

Inhibition effects of paeonol on mice bearing EMT6 breast cancer through inducing rumor cell apoptosis

Paeonol, a phenolic component from the root bark of Paeonia moutan, has been identified to possess antitumor effects on mice bearing EMT6 breast cancer in our previous studies. However, the underlying mechanisms remain unknown. In the present study the molecular mechanisms of paeonol were further investigated in EMT6 mice model. The results showed that treatment of mice with 175 and 350 mg/kg/day of paeonol significantly inhibited the growth of the EMT6 tumor in mice, and induced tumor cell apoptosis which were demonstrated by light microscopy after hematoxylin and eosin staining and apoptosis analysis by flow cytometry. In addition, compared with the control group, paeonol increased the number of tumor cells in G0/G1 phase but decreased the number of cells in S and G2/M phase. Paeonol treatment (350 mg/kg body weight) also resulted in a decrease of Bcl-2 and an increase in Bax and caspase-3 expressions, which were demonstrated by immunohistochemical and western blot analysis. These results indicate that the antitumor effects of paeonol might be associated with arresting tumor cells in the G0/G1 phase, inducing cell apoptosis and regulation of the expression of Bcl-2, Bax and activation of caspase-3

Porcine reproductive and respiratory syndrome virus infection triggers HMGB1 release to promote inflammatory cytokine production

AbstractThe high mobility group box 1 (HMGB1) protein is an endogenous damage-associated molecular pattern (DAMP) molecule involved in the pathogenesis of various infectious agents. Based on meta-analysis of all publicly available microarray datasets, HMGB1 has recently been proposed as the most significant immune modulator during the porcine response to porcine reproductive and respiratory syndrome virus (PRRSV) infection. However, the function of HMGB1 in PRRSV pathogenesis is unclear. In this study, we found that PRRSV infection triggers the translocation of HMGB1 from the nucleus to the extracellular milieu in MARC-145 cells and porcine alveolar macrophages. Although HMGB1 has no effect on PRRSV replication, HMGB1 promotes PRRSV-induced NF-κB activation and subsequent expression of inflammatory cytokines through receptors RAGE, TLR2 and TLR4. Our findings show that HMGB1 release, triggered by PRRSV infection, enhances the efficiency of virus-induced inflammatory responses, thereby providing new insights into the pathogenesis of PRRSV infection

Optimizing energy efficiency of CNN-based object detection with dynamic voltage and frequency scaling

On the one hand, accelerating convolution neural networks (CNNs) on FPGAs requires ever increasing high energy efficiency in the edge computing paradigm. On the other hand, unlike normal digital algorithms, CNNs maintain their high robustness even with limited timing errors. By taking advantage of this unique feature, we propose to use dynamic voltage and frequency scaling (DVFS) to further optimize the energy efficiency for CNNs. First, we have developed a DVFS framework on FPGAs. Second, we apply the DVFS to SkyNet, a state-of-the-art neural network targeting on object detection. Third, we analyze the impact of DVFS on CNNs in terms of performance, power, energy efficiency and accuracy. Compared to the state-of-the-art, experimental results show that we have achieved 38% improvement in energy efficiency without any loss in accuracy. Results also show that we can achieve 47% improvement in energy efficiency if we allow 0.11% relaxation in accuracy

Design and analysis of miniaturized low profile and second-order multi-band polarization selective surface for multipath communication application

In this paper, a novel frequency selective surface (FSS) is designed; it has the characteristics of the low profile, second-order, multi-band, and the remarkable polarization selection properties. In the following, such an FSS having polarization selection characteristics will be referred to simply as a polarization selection surface (PSS). In a specific frequency band, the proposed PSS has a second-order selective transmission characteristic for TE and TM waves. Based on the coupling resonance filtering mechanism, the proposed PSS is composed of three metallic layers separated by two layers of dielectric substrates, which serves as the spatial form of the second-order microwave filter. The proposed PSS uses a sub-wavelength periodic structure array consisting of a non-resonant unit, and the unit size and the period within the range of 0.08λ 1 -0.15λ 1 , where the λ 1 =40.76 mm is the first passband wavelength of free space, so the PSS miniaturization characteristic is remarkable. The theoretical analysis and measure results show that the proposed bandpass PSS has good second-order polarization selection characteristics, out-of-band suppression level, and the flat transmission band, compared with the first-order bandpass PSS. In the range of incident angle of 0°-60°, it has a stable frequency response. It provides a reference for the design of a polarization wave generator and a polarization separation structure in a multipath communication system. © 2019 IEEE

Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain

Background: Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia (EP), a mild, chronic pneumonia of swine. Despite presenting with low direct mortality, EP is responsible for major economic losses in the pig industry. To identify the virulence-associated determinants of M. hyopneumoniae, we determined the whole genome sequence of M. hyopneumoniae strain 168 and its attenuated high-passage strain 168-L and carried out comparative genomic analyses. Results: We performed the first comprehensive analysis of M. hyopneumoniae strain 168 and its attenuated strain and made a preliminary survey of coding sequences (CDSs) that may be related to virulence. The 168-L genome has a highly similar gene content and order to that of 168, but is 4,483 bp smaller because there are 60 insertions and 43 deletions in 168-L. Besides these indels, 227 single nucleotide variations (SNVs) were identified. We further investigated the variants that affected CDSs, and compared them to reported virulence determinants. Notably, almost all of the reported virulence determinants are included in these variants affected CDSs. In addition to variations previously described in mycoplasma adhesins (P97, P102, P146, P159, P216, and LppT), cell envelope proteins (P95), cell surface antigens (P36), secreted proteins and chaperone protein (DnaK), mutations in genes related to metabolism and growth may also contribute to the attenuated virulence in 168-L. Furthermore, many mutations were located in the previously described repeat motif, which may be of primary importance for virulence. Conclusions: We studied the virulence attenuation mechanism of M. hyopneumoniae by comparative genomic analysis of virulent strain 168 and its attenuated high-passage strain 168-L. Our findings provide a preliminary survey of CDSs that may be related to virulence. While these include reported virulence-related genes, other novel virulence determinants were also detected. This new information will form the foundation of future investigations into the pathogenesis of M. hyopneumoniae and facilitate the design of new vaccines

A multifunctional tripodal fluorescent probe for the recognition of Cr3+, Al3+, Zn2+ and F− with controllable ESIPT processes

Three 4-(benzo[d]thiazol-2-yl)-2,5-dihydroxybenzaldehyde fluorophores were introduced to construct a tripodal multifunctional ESPIT fluorescence probe L. The fluorescent analysis revealed that probe L exhibited excellent recognition capabilities towards Cr3+, Al3+, Zn2+ and F− ions with large Stokes shifts. Furthermore, under optimal conditions, the detection limit of probe L towards Cr3+, Al3+, Zn2+ and F− were low, of the order of 10−8 M, which indicated that probe L was sensitive to these four ions. Interestingly, the fluorescent and 1H NMR titration experiments revealed that the recognition mechanism of probe L towards the ions Cr3+, Al3+, Zn2+ and F− were different. The presence of Cr3+ and Al3+ recovered the ESIPT, but the presence of Zn2+ trigger a moderate deprotonation of the phenolic OH and induced an ESIPT red-shifted (60 nm) emission wavelength. Finally, the presence of F− completely deprotonated the free phenolic OH and a remarkable red-shifted (130 nm) ESIPT emission was observed. In other words, the ESIPT process of probe L is controllable. Furthermore, the utility of probe L as a biosensor in living cells (PC3 cells) towards Cr3+, Al3+ and Zn2+ ions has been demonstrated

Search for light dark matter from atmosphere in PandaX-4T

We report a search for light dark matter produced through the cascading decay of $\eta$ mesons, which are created as a result of inelastic collisions between cosmic rays and Earth's atmosphere. We introduce a new and general framework, publicly accessible, designed to address boosted dark matter specifically, with which a full and dedicated simulation including both elastic and quasi-elastic processes of Earth attenuation effect on the dark matter particles arriving at the detector is performed. In the PandaX-4T commissioning data of 0.63 tonne$\cdot$year exposure, no significant excess over background is observed. The first constraints on the interaction between light dark matter generated in the atmosphere and nucleus through a light scalar mediator are obtained. The lowest excluded cross-section is set at $5.9 \times 10^{-37}{\rm cm^2}$ for dark matter mass of $0.1$ MeV$/c^2$ and mediator mass of 300 MeV$/c^2$. The lowest upper limit of $\eta$ to dark matter decay branching ratio is $1.6 \times 10^{-7}$